Rapid detection of Cyanobacteria by recombinase polymerase amplification combined with lateral flow strips |
Li, JJ (Li, Jingjing); Wang, CM (Wang, Chunming); Yu, X (Yu, Xin); Lin, HR (Lin, Huirong); Hui, C (Hui, Chen); Shuai, L (Shuai, Li); Zhang, SH* (Zhang, Shenghua) |
Cyanobacteria are one of the major groups of algae causing algal blooms. In this study, we developed a rapid method for detecting Cyanobacteria using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. After releasing cyanobacterial DNA from cells using a freeze-cracking method, DNA was amplified using the RPA method. Next, the RPA products were detected using the LF test. LF-RPA successfully amplified the DNA of eight cyanobacterial species and detected their presence in the sample with high specificity, distinguishing them from five non-cyanobacterial species. The method could detect cyanobacterial DNA in water samples containing as low as 0.01 cell/mL Cyanobacteria, making the method more sensitive than polymerase chain reaction (PCR), which required cell densities of at least 104 cell/mL. LF-RPA could amplify and detect cyanobacterial DNA at any temperature in the range 30–45 °C in just 30 min and without the need for a thermal cycler. The method developed in this study is simple, rapid, and effective for on-site testing of Cyanobacteria, which may become a routine measurement in efforts to detect and treat harmful algal blooms. |
Key words:cyanobacteria; lateral flow strips; rapid detection; recombinase polymerase amplification |
Volume:19 Page:1181-1186 Journal:WATER SCIENCE AND TECHNOLOGY-WATER SUPPLY |
https://doi.org/10.2166/ws.2018.174
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